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KMID : 0358419750180060395
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Korean Journal of Obstetrics and Gynecology
1975 Volume.18 No. 6 p.395 ~ p.405
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Radioimmunoassay of Serum Testosterone Level in Menstrual Cycle and Androgenic Diseases
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崔成璣/Choi, S.K
金潤浩/羅琮球/鄭炳來/Kim, Y.H/rha, J.K/chung, B.L
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Abstract
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Many methods on the measurement of plasma testosterone have been developed during last decade. Although some of them, such as double isotope dilution and gas-liquid chromatography with electron capture have been known to . be reasonable, accurate, precise, and specific, they are tedious and unpracticable for clinical application. Even though the development of the competitive protein binding assay has made clinical measurement of testosterone comparatively practical, only a few samples can be processed at one time in the assay.
In this study serum testosterone levels of 4 normal women through full menstrual cycle, 4 female patients with various androgenic disorders, and a healthy adult man were measured by modified Midgley (1966) method of double antibody technique using radioimmunoassay kit obtained from Serono Immunochemical Company (Rome, Italy).
The results were as follows.
1) The recovery of testosterone after extraction with ethanol was 89.2 per cent. However, ethanol and benzene was improper as extractive solvent because of difficulty in its separation and evaporation. The recoveries of testosterone with ether, petroleum ether and benzene were 68.9 per cent, 15.6 per cent, and 67.7 per cent, respectively. Thus it was found that ethyl ether is most suitable for extraction of testosterone.
2) The mean serum testosterone level after extraction of female patients with androgenic disorders was 2.06±1.09 fig/ml, while that without extraction was above 4 ng/ml. The difference in testosterone value between before and after extraction was significant (P<0.001) .
3) The mean serum testosterone level through menstrual cycle in normal women was 1.27 ng/ml. The average serum testosterone levels in menstrual phase and premenstrual phase were 0.97 ng/ml and 1.06 ng/ml, respectively. On the other hand, the level in ovulatory phase was 1.96ng/ml, which is significantly ´higher than those in menstrual and premenstrual phases.
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KEYWORD
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